Composite

Part:BBa_K2549016:Design

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-09)


LaG17-mN1c-tTAA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 87
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2154
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 127
    Illegal SapI.rc site found at 1069


Design Notes

LaG17-mN1c-tTAA was assembled by joining LaG17 with a CD8α signal peptide and a Myc tag on its N terminal, mouse Notch1 core and tTA advance together using ClonExpress[1]. LaG17 with a CD8α signal peptide and a Myc tag on its N terminal fragment was synthesized by IDT (gBlock).

Source

This part was assembled by CD8α signal peptide (Part:BBa_K2549044), cMyc-tag (Part:BBa_K823036), LaG17 (Part:BBa_K2549004), mouse Notch1 core (Part:BBa_K2549006) and tTA advanced (Part:BBa_K2446057).

References

  1. ↑ http://www.vazymebiotech.com/products_detail/productId=195.html